Differentiation of motile and mesophilic Aeromonas strains into species by testing for a CAMP-like factor

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Differentiation of motile and mesophilic Aeromonas strains into species by testing for a CAMP-like factor.

Motile and mesophilic Aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a CAMP-like factor. Aeromonas hydrophila strains were positive either aerobically or anaerobically, Aeromonas sobria strains were positive only aerobically, and Aeromonas caviae strains were always negative.

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Congo red uptake by motile Aeromonas species.

Virulence of several species of enteropathogenic bacteria has been correlated with the ability of isolates to take up the dye Congo red. To determine whether Congo red uptake might be a useful marker for virulence of motile Aeromonas species, we examined 50 strains of diverse clinical origin on a medium containing 50 micrograms of Congo red per ml. All of the strains took up the dye to various ...

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A colonization factor (production of lateral flagella) of mesophilic Aeromonas spp. is inactive in Aeromonas salmonicida strains.

The nine laf (lateral flagellum) genes of mesophilic aeromonads are in the Aeromonas salmonicida genome. The laf genes are functional, except for lafA (flagellin gene), which was inactivated by transposase 8 (IS3 family). A pathogenic characteristic of mesophilic aeromonads (lateral flagella) is abolished in this specialized pathogen with a narrow host range.

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Role of flm locus in mesophilic Aeromonas species adherence.

The adherence mechanism of Aeromonas caviae Sch3N to HEp-2 cells was initially investigated through four mini-Tn5 mutants that showed a 10-fold decrease in adherence. These mutants lost motility, flagella, and their lipopolysaccharide (LPS) O antigen (O-Ag). Three genes, flmB-neuA-flmD, were found to be interrupted by the transposon insertions; additionally, two other genes, one lying upstream ...

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Reassessment of the Enteropathogenicity of Mesophilic Aeromonas Species

Cases of Aeromonas diarrhea have been described all over the world. The genus Aeromonas includes ca. 30 species, of which 10 have been isolated in association with gastroenteritis. The dominating species that account for ca. 96% of the identified strains are Aeromonas caviae, A. veronii, A. dhakensis, and A. hydrophila. However, the role of Aeromonas as a true enteropathogen has been questioned...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1987

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.25.7.1341-1342.1987